DMT-2'O-Methyl-rU Phosphoramidite Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 110764-79-9 |
| Product name | DMT-2'O-Methyl-rU Phosphoramidite |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Industrial Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for DMT-2'O-Methyl-rU Phosphoramidite
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk DMT-2'O-Methyl-rU Phosphoramidite Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of DMT-2'O-Methyl-rU Phosphoramidite
| Industry | Application | Process |
|---|---|---|
| Biopharmaceuticals | 2′-O-methyl uridine monomer for solid-phase oligonucleotide synthesis (e.g., siRNA, antisense oligonucleotides) | Used as a phosphoramidite monomer in automated solid-phase oligonucleotide synthesis; incorporated via iterative cycles of deprotection, coupling, oxidation, and capping to achieve sequence-specific chain elongation; the 5′-DMT group serves as a temporary protecting group and enables real-time coupling monitoring; the 2′-O-methyl modification enhances nuclease resistance and target-binding affinity; the 3′-cyanoethyl diisopropylphosphoramidite group ensures high reactivity and good storage stability |
Manufacturing Quality Control of DMT-2'O-Methyl-rU Phosphoramidite
| Test Item | Common Testing Method | Method Overview |
|---|---|---|
| Appearance | Visual Inspection | Observe the sample's color, physical state, and whether caking occurs. The material should normally be a white to off-white powder or amorphous solid, free from visible impurities. |
| Purity (Main Component Content) | High-Performance Liquid Chromatography (HPLC) | Uses a reversed-phase C18 column with gradient elution (e.g., acetonitrile/water containing trifluoroacetic acid or phosphate buffer system) and UV detection (typically at 260 nm). Main peak purity is calculated by peak area normalization or external standard method, generally requiring ≥98%. |
| Structural Confirmation | Nuclear Magnetic Resonance Spectroscopy (NMR) (¹H NMR, ¹³C NMR, ³¹P NMR) |
Analyzes chemical shifts, coupling constants, and integration ratios of hydrogen, carbon, and phosphorus atoms to confirm the presence and connectivity of functional groups in the molecular structure, especially characteristic signals of DMT protecting group, 2'-O-methyl, and phosphoramidite moieties. |
| Molecular Weight Confirmation | Mass Spectrometry (MS) (e.g., ESI-MS or MALDI-TOF) |
Detects the protonated molecular ion [M+H]⁺ or sodium adduct [M+Na]⁺ in positive ion mode using electrospray ionization mass spectrometry (ESI-MS), comparing with theoretical molecular weight to verify the molecular composition of the target compound. |
| Moisture Content | Karl Fischer Titration | Measures total water content including both free and bound water. Controls moisture levels to ensure stability of phosphoramidite compounds, typically requiring ≤0.5%. |
| Residual Solvents | Gas Chromatography (GC) | Detects potentially residual organic solvents (e.g., acetonitrile, dichloromethane, tetrahydrofuran, toluene) according to ICH guidelines, using appropriate columns and detectors (FID), ensuring compliance with specified limits. |
| Chiral Purity / Enantiomeric Impurities | Chiral High-Performance Liquid Chromatography (Chiral HPLC) | If necessary, evaluates stereochemical purity at chiral centers (e.g., phosphoramidite moiety) to prevent diastereomeric impurities that could affect downstream synthesis efficiency. |
| Assay | UV-Vis Spectrophotometry combined with HPLC External Standard Method | Utilizes the characteristic absorbance of the uracil ring at 260 nm along with a standard curve to quantify total nucleoside derivative content; or employs HPLC external standard method for accurate determination of active ingredient concentration. |
| Heavy Metal Residues | Inductively Coupled Plasma Mass Spectrometry (ICP-MS) or Atomic Absorption Spectroscopy (AAS) | Detects toxic metal residues such as lead, mercury, cadmium, and arsenic, ensuring compliance with pharmacopeial or GMP permissible limits. |
| Microbial Limits (if used in pharmaceuticals) | Microbial Limit Test | Performs total aerobic microbial count, mold and yeast count, and tests for specified microorganisms according to pharmacopeial requirements; applicable for quality control when used as an intermediate in active pharmaceutical ingredient (API) manufacturing. |
Technical Specifications of DMT-2'O-Methyl-rU Phosphoramidite
| Test Item | Specification |
|---|---|
| Appearance | White to off-white powder |
| Purity | >=98.0% |
| Total Impurities | <=2.0% |
| Single Impurity | <=0.5% |
| Water Content (KF) | <=0.5% |
| Identification (NMR) | Consistent with structure |
| Optical Rotation | -15.0 to -25.0 deg (c=1, CHCl3) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of DMT-2'O-Methyl-rU Phosphoramidite
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
NMR Spectra of DMT-2'O-Methyl-rU Phosphoramidite
Safety Information of DMT-2'O-Methyl-rU Phosphoramidite
| Pictograms |  |
| Signal Word | Warning |
| Safety Data Sheet | |
Packaging of DMT-2'O-Methyl-rU Phosphoramidite
Industrial DMT-2'O-Methyl-rU Phosphoramidite Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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