2’-O-Methyl-rA(N-Bz)phosphoramidite Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 110782-31-5 |
| Product name | 2’-O-Methyl-rA(N-Bz)phosphoramidite |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Industrial Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for 2’-O-Methyl-rA(N-Bz)phosphoramidite
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk 2’-O-Methyl-rA(N-Bz)phosphoramidite Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of 2’-O-Methyl-rA(N-Bz)phosphoramidite
| Industry | Application | Process |
|---|---|---|
| Biopharmaceuticals | Adenosine-modified monomers for solid-phase oligonucleotide synthesis (e.g., siRNA, antisense oligonucleotides) | Used as phosphoramidite monomers in DNA/RNA synthesizers; incorporated into nucleic acid chains containing 2′-O-methyl modifications via cyclic coupling, oxidation, and deprotection steps |
Manufacturing Quality Control of 2’-O-Methyl-rA(N-Bz)phosphoramidite
| Test Item | Common Testing Methods | Method Overview |
|---|---|---|
| Appearance | Visual Inspection | Observe the color, physical state, and whether the sample is clumped. Qualified products are usually white to off-white powder, with no visible impurities. |
| Purity (Main Component Content) | High-Performance Liquid Chromatography (HPLC) | Use a reversed-phase C18 column with gradient elution (e.g., acetonitrile/water containing trifluoroacetic acid or ammonium acetate buffer), detected by UV (~260 nm). The purity of the main peak is calculated using area normalization or external standard method, typically requiring ≥98%. |
| Moisture Content | Karl Fischer Titration | Determine the total amount of bound water and free water. Particularly important for phosphoramidite compounds, as excessive moisture can lead to reduced activity. Generally requires ≤0.5%. |
| Residual Solvents | Gas Chromatography (GC) | Detect possible residual organic solvents (such as acetonitrile, toluene, dichloromethane, etc.) during synthesis. Limits are set according to ICH Q3C guidelines, using capillary columns and FID detectors. |
| Chiral Purity / Enantiomeric Impurities | Chiral High-Performance Liquid Chromatography (Chiral HPLC) | If there are chiral centers (such as sugar ring structures), assess enantiomeric excess (ee value). Separate and detect using chiral stationary phase columns. |
| Phosphoramidite Activity (Coupling Efficiency) | Nuclear Magnetic Resonance Spectroscopy (³¹P NMR) or Actual Synthesis Test | Analyze the characteristic peak chemical shift of phosphoramidite (usually ~149–151 ppm) by ³¹P NMR to determine structural correctness and presence of oxidation by-products (phosphate ester peak ~0–5 ppm). Alternatively, perform small-scale coupling experiments using an automated DNA/RNA synthesizer to measure coupling efficiency (should be >98%). |
| Structure Confirmation | Nuclear Magnetic Resonance Spectroscopy (¹H NMR, ¹³C NMR, ³¹P NMR), Mass Spectrometry (MS) | Confirm molecular structure, substitution positions, and molecular ion peaks matching theoretical molecular weight by combining multi-nucleus NMR and high-resolution mass spectrometry (HRMS or ESI-MS). |
| Content Determination | UV-Vis Spectrophotometry Combined with HPLC External Standard Method | Quantify the total nucleoside derivative content using the characteristic absorption of adenosine at 260 nm, combined with a standard curve; or accurately determine the concentration of active ingredients using the HPLC external standard method. |
| Microbial Limit (if applicable) | Microbial Limit Test | If used for pharmaceutical-grade oligonucleotide synthesis, test for bacterial and mold counts and control organisms (such as Escherichia coli, Salmonella, etc.), following pharmacopoeia methods. Generally, it is not a sterile raw material, so this item may not be required. |
| Ignited Residue / Heavy Metals | Ignited Residue Method + Heavy Metal Test (e.g., Atomic Absorption Spectroscopy AAS or ICP-MS) | Determine inorganic impurity residues. The residue weight after ignition is measured (generally ≤0.1%); the total heavy metal content is generally required to be ≤10 ppm. |
Technical Specifications of 2’-O-Methyl-rA(N-Bz)phosphoramidite
| Test Item | Specification |
|---|---|
| Appearance | White solid |
| Purity | >=98.0% (HPLC) |
| Total Impurities | <=2.0% (HPLC) |
| Single Impurity | <=0.5% (HPLC) |
| Water Content (KF) | <=0.5% |
| Identification (NMR) | Consistent with structure |
| Optical Rotation | -15.0° to -25.0° (c=1, in CHCl3) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of 2’-O-Methyl-rA(N-Bz)phosphoramidite
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
NMR Spectra of 2’-O-Methyl-rA(N-Bz)phosphoramidite
Safety Information of 2’-O-Methyl-rA(N-Bz)phosphoramidite
| Pictograms |  |
| Signal Word | Warning |
| Safety Data Sheet | |
Packaging of 2’-O-Methyl-rA(N-Bz)phosphoramidite
Industrial 2’-O-Methyl-rA(N-Bz)phosphoramidite Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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