D-AMINO ACID OXIDASE Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 9000-88-8 |
| Product name | D-AMINO ACID OXIDASE |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeReagent GradeFood Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for D-AMINO ACID OXIDASE
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk D-AMINO ACID OXIDASE Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of D-AMINO ACID OXIDASE
| Industry | Application | Process |
|---|---|---|
| Pharmaceuticals | Detection and quantitative analysis of D-amino acids for auxiliary diagnosis of metabolic disorders (e.g., D-aminoaciduria) | Enzymatic assay: D-amino acid oxidase catalyzes the oxidation of D-amino acids to α-keto acids, ammonia, and hydrogen peroxide; the latter is detected colorimetrically or fluorometrically via coupled reactions |
| Pharmaceutical Manufacturing | Synthesis of chiral drug intermediates; removal of D-configured impurities from racemic mixtures | Kinetic resolution: selective oxidation of D-amino acids, retaining the L-configuration for synthesis of chiral pharmaceuticals (e.g., semi-synthetic penicillins and cephalosporins) |
| Food | Quantification of D-amino acid content in food products to assess extent of protein hydrolysis or detect adulteration | Enzyme-based sensor or colorimetric assay: D-amino acid oxidase selectively recognizes D-amino acids, with quantification achieved via electrochemical or optical signal output |
| Biotechnology | Construction of biocatalytic systems for asymmetric synthesis or biotransformation | Immobilized enzyme reactor: D-amino acid oxidase immobilized on a support matrix for continuous production of high-purity L-amino acids or related derivatives |
| Research | Investigation of amino acid metabolic pathways and redox mechanisms | In vitro enzymology: used as a tool enzyme to study metabolic regulation of D-amino acids in microorganisms or mammalian systems |
Manufacturing Quality Control of D-AMINO ACID OXIDASE
| Detection Item | Common Analytical Method | Method Overview |
|---|---|---|
| Enzyme Activity | Spectrophotometric Assay (UV-Vis) | Measures H2O2 production rate at 240 nm or coupled peroxidase reaction at 500 nm; activity calculated from NADH oxidation or chromogen formation. |
| Protein Concentration | Bradford Assay | Based on Coomassie Brilliant Blue G-250 dye binding to basic and aromatic amino acid residues, causing spectral shift measurable at 595 nm. |
| Purity (SDS-PAGE) | SDS-Polyacrylamide Gel Electrophoresis | Separates denatured protein subunits by molecular weight; purity assessed via single band intensity and absence of contaminating bands. |
| Residual Host Cell Proteins | ELISA | Quantifies non-target mammalian or bacterial proteins using antigen-specific antibodies and enzymatic colorimetric detection. |
| Endotoxin Level | LAL Assay (Turbidimetric) | Uses Limulus amebocyte lysate to detect endotoxin-induced coagulation cascade, measured as turbidity increase at 340 nm. |
| Residual DNA | Fluorometric DNA Quantitation | Employs DNA-binding dyes (e.g., PicoGreen) with selective fluorescence enhancement upon dsDNA binding, measured at excitation/emission ~480/520 nm. |
| Moisture Content | Karl Fischer Titration | Determines water content via stoichiometric iodine-sulfur dioxide reaction in methanol-based solvent, monitored potentiometrically or coulometrically. |
| pH (Solution) | Potentiometric pH Measurement | Measures hydrogen ion activity using calibrated glass electrode and reference electrode in aqueous suspension or reconstituted solution. |
| Heavy Metals | ICP-OES | Quantifies trace metal impurities (e.g., Pb, Cd, Ni) by atomization and excitation in argon plasma, followed by elemental emission line detection. |
| Microbial Bioburden | Membrane Filtration and Plate Count | Filters sample through sterile membrane, transfers to solid agar media, incubates, and enumerates viable colony-forming units. |
| Sterility | Direct Inoculation into Culture Media | Inoculates product into fluid thioglycollate and soybean-casein digest media, incubates for 14 days, and observes for microbial growth. |
| Specific Absorbance Ratio (A280/A260) | UV-Vis Spectrophotometry | Evaluates nucleic acid contamination by comparing absorbance at 280 nm (protein) and 260 nm (nucleic acids); ratio >1.8 indicates low nucleic acid content. |
Technical Specifications of D-AMINO ACID OXIDASE
| Test Item | Specification |
|---|---|
| Appearance | White to off-white lyophilized powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.5% |
| Optical Rotation | -25.0 to -35.0 deg (c=1, H2O) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of D-AMINO ACID OXIDASE
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
Packaging of D-AMINO ACID OXIDASE
Industrial D-AMINO ACID OXIDASE Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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