Ribonuclease A Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 9001-99-4 |
| Product name | Ribonuclease A |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeReagent Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for Ribonuclease A
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk Ribonuclease A Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of Ribonuclease A
| Industry | Application | Process |
|---|---|---|
| Biomedical Research | RNA degradation, RNA structural and functional studies, removal of RNA contamination | Added to samples in molecular biology experiments to specifically hydrolyze RNA; commonly applied in plasmid DNA isolation, pre-RT-PCR treatment, and ribosome profiling. |
| Diagnostics Development | Used as a positive control or activity reference standard in RNase detection assay kits | In vitro enzyme activity assays, where RNase A at a known concentration serves as the reference standard to quantify test sample RNase activity via spectrophotometric (e.g., absorbance change at 260 nm following RNA substrate degradation) or fluorometric methods. |
| Cell Biology | Induction of apoptosis studies, regulation of non-coding RNA metabolism | Delivered into the cytoplasm via permeabilization to trigger RNA degradation–dependent apoptotic pathways; applied to investigate the impact of tRNA/rRNA fragmentation on cellular stress responses. |
| Biopharmaceutical Quality Control | Detection of residual RNA in manufacturing environments or evaluation of nucleic acid clearance process efficacy | Used as a challenge agent to validate the removal efficiency of RNA clearance steps (e.g., alkaline treatment, thermal inactivation, filtration) during the production of sterile formulations, viral vectors, or mRNA vaccines. |
| Food Science (Research Use) | Model enzyme for evaluating RNA stability | Employed as a tool enzyme under simulated digestion or processing conditions to measure the in vitro degradation kinetics of functional RNA (e.g., miRNA) in food matrices, thereby assessing its potential bioavailability. |
Manufacturing Quality Control of Ribonuclease A
| Detection Item | Common Analytical Method | Method Overview |
|---|---|---|
| Protein Concentration | UV-Vis Spectrophotometry | Measures absorbance at 280 nm using the extinction coefficient to quantify protein concentration based on aromatic amino acid content. |
| Enzymatic Activity | Spectrophotometric Assay (using cCMP substrate) | Monitors decrease in absorbance at 296 nm as RNase A hydrolyzes cytidine 2',3'-cyclic monophosphate (cCMP) to 3'-CMP. |
| Purity (by SDS-PAGE) | SDS-Polyacrylamide Gel Electrophoresis | Separates proteins by molecular weight under denaturing conditions; purity assessed via single band intensity and absence of contaminating bands. |
| Residual Moisture | Karl Fischer Titration | Quantifies water content via coulometric or volumetric titration with iodine, based on the stoichiometric reaction between iodine and water. |
| pH | Potentiometric pH Measurement | Determines hydrogen ion activity using a calibrated glass electrode and reference electrode in aqueous solution. |
| Endotoxin Level | Limulus Amebocyte Lysate (LAL) Assay | Detects bacterial endotoxins via enzymatic cascade activation in horseshoe crab lysate, measured turbidimetrically or chromogenically. |
| Heavy Metals | ICP-OES | Quantifies trace metal impurities (e.g., Pb, Cd, Hg, As) by measuring element-specific atomic emission after plasma excitation. |
| Residual Host Cell Proteins | ELISA | Uses antibody-based capture and detection to quantify non-RNase A mammalian or bacterial host cell proteins. |
| Bioburden | Membrane Filtration and Colony Counting | Retains viable microorganisms from sample on membrane filter, followed by incubation on agar media and enumeration of colony-forming units. |
| Appearance | Visual Inspection | Assesses physical state (lyophilized powder or solution), color, and clarity against defined standards under controlled lighting. |
| Specific Activity | Enzymatic Activity / Protein Concentration Ratio | Calculates activity units per mg protein, derived from independent UV-Vis and cCMP assay results to confirm functional integrity. |
| Sterility | Membrane Filtration with Incubation (USP <71>) | Filters sample through sterile membrane, transfers to fluid thioglycollate and soybean-casein digest media, and incubates for microbial growth detection. |
Technical Specifications of Ribonuclease A
| Test Item | Specification |
|---|---|
| Appearance | White to off-white lyophilized powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.3% |
| Optical Rotation | -50.0° to -60.0° (c=1, H2O) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of Ribonuclease A
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
Packaging of Ribonuclease A
Industrial Ribonuclease A Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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