PHOSPHODIESTERASE I Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 9025-82-5 |
| Product name | PHOSPHODIESTERASE I |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeReagent Grade |
| Packaging | 25KG/bag500KG/bag1000KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for PHOSPHODIESTERASE I
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk PHOSPHODIESTERASE I Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of PHOSPHODIESTERASE I
| Industry | Application | Process |
|---|---|---|
| Biopharmaceuticals | Used for exonuclease activity analysis in DNA/RNA sequencing, gene cloning, and molecular biology research | Catalyzes the hydrolysis of single-stranded or double-stranded DNA/RNA from the 5′ end to generate 5′-mononucleotides in vitro; commonly applied in labeled probe preparation or nucleic acid template degradation |
| Diagnostics | Serves as a critical component in enzyme-linked immunosorbent assays (ELISA) or nucleic acid detection | Participates in signal amplification or background nucleic acid removal by specifically cleaving phosphodiester bonds |
| Research Tools | Employed in nucleic acid structure–function studies and nuclease mechanism elucidation | Performs targeted degradation of specific nucleic acid substrates under controlled buffer conditions to support structural analysis or enzyme kinetic studies |
Manufacturing Quality Control of PHOSPHODIESTERASE I
| Detection Item | Common Analytical Method | Method Overview |
|---|---|---|
| Enzyme Activity | Spectrophotometric Assay (pNPP substrate) | Measures hydrolysis rate of p-nitrophenyl phosphate to p-nitrophenol at 405 nm, proportional to enzymatic activity. |
| Protein Content | Bradford Assay | Based on binding of Coomassie Brilliant Blue G-250 dye to basic and aromatic amino acid residues, with absorbance measured at 595 nm. |
| Purity (SDS-PAGE) | Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis | Separates proteins by molecular weight under denaturing conditions; purity assessed by single dominant band intensity. |
| Residual Host Cell Proteins | ELISA | Quantifies non-target host cell proteins using antibody-based capture and enzymatic colorimetric detection. |
| Residual DNA | Fluorometric Assay (PicoGreen) | Uses DNA-binding fluorescent dye with excitation/emission at 480/520 nm; signal intensity correlates with double-stranded DNA concentration. |
| Heavy Metals (Pb, Cd, Hg, As) | ICP-MS | Ionizes sample in plasma and detects elemental isotopes by mass-to-charge ratio with high sensitivity and multi-element capability. |
| Moisture Content | Karl Fischer Titration | Measures water via stoichiometric reaction between iodine, sulfur dioxide, methanol, and water in anhydrous medium. |
| pH (1% w/v solution) | Potentiometric Measurement | Determines hydrogen ion activity using calibrated glass electrode and reference electrode in aqueous suspension. |
| Microbial Contamination | Membrane Filtration and Plate Count | Filters sample through sterile membrane, transfers to growth media, and enumerates viable aerobic mesophilic microorganisms after incubation. |
| Endotoxin Level | Kinetic Turbidimetric LAL Assay | Measures endotoxin-induced coagulation cascade in Limulus amebocyte lysate, quantified by rate of turbidity development. |
| Specific Activity | Calculated from Enzyme Activity and Protein Content | Ratio of measured enzyme activity (U/mg) to total protein concentration determined by Bradford or similar method. |
| Appearance and Solubility | Visual Inspection and Dissolution Test | Assesses physical form (lyophilized powder or solid), color, and complete dissolution in specified buffer within defined time. |
Technical Specifications of PHOSPHODIESTERASE I
| Test Item | Specification |
|---|---|
| Appearance | White to off-white lyophilized powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.5% |
| Solubility | Freely soluble in water; insoluble in organic solvents such as acetone and ethanol |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of PHOSPHODIESTERASE I
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
Packaging of PHOSPHODIESTERASE I
Industrial PHOSPHODIESTERASE I Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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