China Factory Direct
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| CAS number | 9066-59-5 |
| Product name | Lysozyme hydrochloride |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeFood GradeCosmetic Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
Based on current marketvolatility and material availability.
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
| Industry | Application | Process |
|---|---|---|
| Pharmaceuticals | Used as a stabilizer and pH adjuster for lysozyme in the preparation of lysozyme injection solutions, ophthalmic solutions, and other formulations | Dissolve lysozyme hydrochloride in water for injection under aseptic conditions, sterilize by filtration through a 0.22 μm membrane filter, and fill into sterile containers |
| Food | Used as a food-grade preservative and freshness enhancer to inhibit growth of Gram-positive bacteria and extend shelf life of dairy and egg products | Add at 0.01–0.1% (w/w) to liquid milk, cheese, or egg liquids prior to pasteurization; maintain pH at 5.5–6.5 to preserve lysozyme activity |
| Biotechnology | Used as a component of cell lysis buffers to enhance lysozyme-mediated hydrolysis efficiency against bacterial cell walls—particularly those of Gram-positive bacteria | Add lysozyme to Tris-HCl or phosphate buffer to a final concentration of 1–10 mg/mL, adjust pH to 6.0–7.0 with hydrochloric acid, and incubate at 37°C for 15–30 minutes to achieve efficient lysis |
| Cosmetics | Used as a mild antimicrobial agent and keratin regulator in acne-treating and oil-control skincare products | Formulate into gels or emulsions with glycerin, hyaluronic acid, etc., at pH 5.0–6.0 and lysozyme concentration of 0.005–0.05% (w/w); avoid high-temperature sterilization to retain enzymatic activity |
| Detection Item | Common Analytical Method | Method Overview |
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| Assay (Lysozyme Activity) | Enzymatic Activity Assay (Micrococcus lysodeikticus Lysis) | Measures enzymatic activity by monitoring turbidity decrease of Micrococcus lysodeikticus cell suspension at 450 nm. |
| Protein Content | UV-Vis Spectrophotometry (280 nm) | Quantifies protein based on absorbance of aromatic amino acids (Trp, Tyr) at 280 nm using extinction coefficient. |
| pH | Potentiometric Titration (pH Meter) | Determines hydrogen ion concentration using calibrated glass electrode in aqueous solution. |
| Residual Solvents | Gas Chromatography (GC-FID) | Separates and quantifies volatile organic solvents based on retention time and flame ionization detection. |
| Heavy Metals (Pb, Cd, Hg, As) | ICP-OES | Simultaneously detects trace metal elements by measuring characteristic atomic emission spectra after plasma excitation. |
| Chloride Ion (Cl-) | Argentometric Titration (Mohr Method) | Titrates chloride with standardized silver nitrate solution using potassium chromate indicator for endpoint detection. |
| Loss on Drying (LOD) | Gravimetric Analysis (Thermogravimetry) | Measures mass loss after drying at 105 C to determine volatile content including water and residual solvents. |
| Microbial Contamination | Membrane Filtration and Plate Count | Filters sample through membrane, incubates on appropriate agar media, and enumerates colony-forming units. |
| Endotoxin Level | Limulus Amebocyte Lysate (LAL) Assay | Detects bacterial endotoxins via coagulation cascade activation in horseshoe crab lysate, measured turbidimetrically or chromogenically. |
| Related Proteins/Impurities | SDS-PAGE | Separates proteins by molecular weight under denaturing conditions; visualized by Coomassie staining to assess purity and degradation. |
| Residual Host Cell Proteins | ELISA | Uses antibody-based capture and detection to quantify host cell protein impurities specific to production system. |
| Sterility | Membrane Filtration and Incubation (USP <71>) | Filters sample under aseptic conditions into fluid thioglycollate and soybean-casein digest media, followed by incubation and growth observation. |
| Test Item | Specification |
|---|---|
| Appearance | White to off-white crystalline powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.5% |
| Optical Rotation | -35.0 to -45.0 deg (c=1, H2O) |
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| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.