β-LactaMase Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 9073-60-3 |
| Product name | β-LactaMase |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeReagent Grade |
| Packaging | 25KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for β-LactaMase
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk β-LactaMase Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of β-LactaMase
| Industry | Application | Process |
|---|---|---|
| Pharmaceutical R&D | Used to study antibiotic resistance mechanisms, particularly the degradation of β-lactam antibiotics (e.g., penicillins, cephalosporins) | Added to in vitro assays to simulate bacterial resistance by introducing β-lactamase, enabling evaluation of novel antibiotics or inhibitors |
| Clinical Diagnostics | Detection of bacterial β-lactamase production to support assessment of pathogen susceptibility to antibiotics | Employed as a control or detection reagent in disk diffusion tests, chromogenic substrate assays, or automated microbiology analysis systems |
| Biotechnology | Utilized as a selection marker or tool enzyme in molecular cloning and protein expression systems | Applied in ampicillin-containing growth media to screen for successfully transformed bacterial clones via the β-lactamase gene (e.g., bla) |
| Pharmaceutical Manufacturing | Used for potency determination and quality control of β-lactamase inhibitors (e.g., clavulanic acid, sulbactam) | Enzyme activity assay: inhibitor efficacy assessed by monitoring the hydrolysis rate of β-lactam substrates |
| Academic Research & Teaching | Serves as a model enzyme for teaching enzyme kinetics, antibiotic mechanisms of action, and resistance principles | Enzyme activity measured via colorimetric or spectrophotometric methods in biochemistry or microbiology laboratory courses |
Manufacturing Quality Control of β-LactaMase
| Detection Item | Common Analytical Method | Method Overview |
|---|---|---|
| Enzyme Activity | Spectrophotometric Assay (UV-Vis) | Measures hydrolysis rate of nitrocefin substrate by monitoring absorbance change at 482 nm |
| Protein Concentration | Bradford Assay | Quantifies protein via Coomassie Brilliant Blue G-250 dye binding and absorbance at 595 nm |
| Purity (SDS-PAGE) | Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis | Resolves protein bands by molecular weight; purity assessed by single dominant band intensity |
| Residual Host Cell Proteins | ELISA | Detects non-target proteins using antibody-based immunoassay with colorimetric readout |
| Endotoxin Level | Limulus Amebocyte Lysate (LAL) Assay | Measures endotoxin via coagulation cascade activation in horseshoe crab lysate |
| Residual DNA | Fluorometric DNA Quantitation | Uses DNA-binding fluorescent dyes (e.g., PicoGreen) and fluorescence intensity measurement |
| Moisture Content | Karl Fischer Titration | Determines water content by volumetric or coulometric titration with iodine-based reagent |
| pH | Potentiometric pH Measurement | Measures hydrogen ion activity using calibrated glass electrode and pH meter |
| Heavy Metals | Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) | Quantifies metal impurities (e.g., Pb, Cd, Hg) by atomic emission after plasma atomization |
| Microbial Contamination | Membrane Filtration and Colony Counting | Detects viable bacteria/fungi by filtration, membrane transfer to agar, and colony enumeration |
| Sterility | Direct Inoculation Sterility Test | Confirms absence of viable microorganisms by incubating sample in fluid thioglycollate and soybean-casein digest media |
| Specific Activity | Combined Bradford and Enzyme Activity Assays | Calculates units of activity per mg protein using parallel protein concentration and activity measurements |
Technical Specifications of β-LactaMase
| Test Item | Specification |
|---|---|
| Appearance | White to off-white lyophilized powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.5% |
| Solubility | Freely soluble in water (10 mg/mL, clear solution) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of β-LactaMase
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
Packaging of β-LactaMase
Industrial β-LactaMase Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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