5,5′-Dithiobis(2-nitrobenzoic acid) Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 69-78-3 |
| Product name | 5,5′-Dithiobis(2-nitrobenzoic acid) |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Pharmaceutical GradeFood GradeCosmetic Grade |
| Packaging | 25KG/bag500KG/bag1000KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for 5,5′-Dithiobis(2-nitrobenzoic acid)
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk 5,5′-Dithiobis(2-nitrobenzoic acid) Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of 5,5′-Dithiobis(2-nitrobenzoic acid)
| Industry | Application | Process |
|---|---|---|
| Biochemistry and Molecular Biology | Quantitative determination of thiol (-SH) groups, for analysis of free cysteine residues in proteins and peptides | Ellman’s assay: DTNB reacts with thiols to generate yellow 5-thio-2-nitrobenzoic acid (TNB⁻), quantified by spectrophotometric measurement at 412 nm |
| Pharmaceutical R&D | As an enzyme activity inhibitor or probe to study the function and regulatory mechanisms of thiol-containing enzymes (e.g., protein tyrosine phosphatases, cathepsin B) | In vitro enzyme kinetic assays, using DTNB as a competitive or non-competitive inhibitor or as an active-site labeling reagent |
| Clinical Diagnostics | Detection of total thiols, reduced glutathione (GSH), and homocysteine in biological samples such as blood and urine | After appropriate dilution or deproteinization, samples are reacted with DTNB buffer and analyzed quantitatively by spectrophotometry |
| Food Science | Assessment of protein thiol oxidation extent and disulfide bond formation status during food processing (e.g., in dairy products and fermented meat products) | Thiols are extracted from homogenized samples using Tris-glycine buffer; free and total thiols are quantified via DTNB colorimetric assay |
| Cosmetic R&D | Monitoring stability of thiol-containing active ingredients (e.g., cysteine derivatives) and redox balance within formulations | During accelerated storage testing, thiol content is tracked periodically using the DTNB assay |
Manufacturing Quality Control of 5,5′-Dithiobis(2-nitrobenzoic acid)
| Detection Item | Common Analytical Method | Method Overview |
|---|---|---|
| Assay (Purity) | UV-Vis Spectrophotometry | Quantifies DTNB concentration based on molar absorptivity at 412 nm in alkaline buffer solution. |
| Loss on Drying | Gravimetric Analysis | Measures mass loss after drying at 105 C under vacuum to determine volatile content. |
| Residue on Ignition | Gravimetric Analysis | Determines inorganic ash content by high-temperature incineration followed by weighing. |
| Heavy Metals (as Pb) | Atomic Absorption Spectroscopy (AAS) | Quantifies total heavy metals by flame or graphite furnace AAS after acid digestion. |
| Chloride Impurity | Potentiometric Titration | Measures chloride ions using silver nitrate titrant with silver indicator electrode. |
| Sulfate Impurity | Gravimetric Analysis | Precipitates sulfate as barium sulfate and determines mass after filtration and drying. |
| Related Substances | HPLC with UV Detection | Separates and quantifies structurally related impurities using reversed-phase chromatography. |
| Melting Point | Capillary Melting Point Apparatus | Determines temperature range of solid-to-liquid transition using calibrated capillary method. |
| Solubility in Water | Visual Observation | Assesses complete dissolution in specified volume of water at defined temperature and time. |
| pH of Aqueous Solution | pH Meter | Measures hydrogen ion activity in a saturated aqueous suspension at 25 C. |
| Particle Size Distribution | Laser Diffraction | Analyzes size distribution of solid particles suspended in liquid medium via light scattering. |
| Bulk Density | Graduated Cylinder Method | Calculates mass per unit volume of freely settled powder in standardized cylinder. |
Technical Specifications of 5,5′-Dithiobis(2-nitrobenzoic acid)
| Test Item | Specification |
|---|---|
| Appearance | Pale-yellow solid |
| Purity | >=98.0% (HPLC) |
| Melting Point | 178-182°C |
| Total Impurities | <=2.0% (HPLC) |
| Single Impurity | <=0.5% (HPLC) |
| Water Content (KF) | <=0.5% |
| Heavy Metals | <=20 ppm (as Pb) |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of 5,5′-Dithiobis(2-nitrobenzoic acid)
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
NMR Spectra of 5,5′-Dithiobis(2-nitrobenzoic acid)
Safety Information of 5,5′-Dithiobis(2-nitrobenzoic acid)
| Pictograms |  |
| Signal Word | Warning |
| Safety Data Sheet | |
Packaging of 5,5′-Dithiobis(2-nitrobenzoic acid)
Industrial 5,5′-Dithiobis(2-nitrobenzoic acid) Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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