Trypan Blue Bulk Supply for Industrial Use
China Factory Direct
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| CAS number | 72-57-1 |
| Product name | Trypan Blue |
| Stock | Stock readily available; annual supply capacity exceeding 1,000 tons. |
| Grade | Industrial Grade |
| Packaging | 25KG/bag500KG/bag1000KG/bag |
| Sample | Test Samples Available (Click to Request Samples) |
| COA | View COA |
| Price | Get Quote |
| Supply Type | Bulk / Contract manufacturing / OEM |
| Shipping | FCL / LCL available |
| Payment Terms | Support FOB \ CIF \ EXW quotations, and accept Letter of Credit (L/C). |
| Manufacturer | Greenrock Chemical(China Factory) |
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Documents We Provide for Trypan Blue
Post-sale: Full documentation is provided.
To request documents, please contact us via email at sales@GRchemical.com.
Bulk Trypan Blue Supplier from China
17+ years in chemical exports.Fully compliant, reliable supply.Your B2B partner in bulk chemical procurement.
Industrial Applications of Trypan Blue
| Industry | Application | Process |
|---|---|---|
| Biopharmaceuticals | Cell viability assessment and cell counting | Add trypan blue stain to the cell suspension; dead cells with compromised membrane integrity take up the dye and appear blue, while viable cells exclude the dye—viability is determined microscopically using a hemocytometer |
| Clinical Diagnostics | Erythrocyte sedimentation rate (ESR) assay support | Used as an anticoagulant additive or suspension medium in ESR testing to prevent erythrocyte aggregation and facilitate measurement of erythrocyte sedimentation distance over one hour |
| Laboratory Research | Cell sorting and cytotoxicity screening | Leverage differential staining of live versus dead cells in combination with flow cytometry or micromanipulation techniques to isolate viable cells for downstream culture or to evaluate drug-induced cytotoxicity |
| Biology Education | Microscopic observation and tracing | Serves as a tracer dye in cell biology experiments for teaching cell morphology, viability assessment, and plasma membrane integrity |
Manufacturing Quality Control of Trypan Blue
| Test Item | Common Testing Method | Method Overview |
|---|---|---|
| Appearance and Color | Visual Inspection | Observe whether the sample is a uniform blue powder or solution, free from clumping, foreign particles, or significant discoloration, consistent with the standard material characteristics. |
| Solubility | Solubility Test | Dissolve trypan blue in water or buffer solution and observe the dissolution rate and clarity of the solution to evaluate complete dissolution and absence of precipitation. |
| Maximum Absorption Wavelength (λmax) | UV-Visible Spectrophotometry (UV-Vis) | Prepare a solution of known concentration and scan between 200–800 nm to confirm that the maximum absorption peak is around 590–605 nm. |
| Absorbance / Concentration Determination | Spectrophotometry (Colorimetric Method) | Use the known molar extinction coefficient (e.g., ε ≈ 6.4×10⁴ L·mol⁻¹·cm⁻¹ at 607 nm) to calculate the actual concentration from absorbance, assessing purity and conformity with labeled specifications. |
| pH Value | pH Meter Measurement | Prepare a solution of specified concentration (e.g., 0.4%) and measure its pH using a calibrated pH meter; the value should typically be within the range of 6.0–7.5. |
| Purity (Impurity Detection) | High-Performance Liquid Chromatography (HPLC) | Analyze main peak purity by HPLC to detect possible synthesis by-products or degradation impurities, evaluating whether sample purity meets required standards. |
| Sterility | Sterility Test (Microbial Limit Test) | Perform sterility testing according to the Chinese Pharmacopoeia or relevant standards to ensure absence of bacterial and fungal contamination; applicable for cell-grade reagents. |
| Endotoxin Content | Limulus Amebocyte Lysate Test (LAL Test) | Detect levels of bacterial endotoxins in the sample; commonly used for trypan blue intended for cell culture, requiring very low endotoxin levels (e.g., <1 EU/mL). |
| Cell Staining Performance Verification | Live/Dead Cell Staining Test | Apply trypan blue to a cell suspension of known viability (e.g., HeLa cells) and observe staining under a microscope to evaluate its ability to selectively stain dead cells. |
Technical Specifications of Trypan Blue
| Test Item | Specification |
|---|---|
| Appearance | Dark blue crystalline powder |
| Purity | >=98.5% |
| Water Content (KF) | <=0.5% |
| Total Impurities | <=1.5% |
| Single Impurity | <=0.5% |
| Heavy Metals | <=20 ppm |
| Residue on Ignition | <=0.3% |
| Melting Point | 175-180 °C |
Specifications vary by industry. Reach out to our technical experts for a COA tailored specifically to your application. Email:selse@GRchemical.com
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Download COA of Trypan Blue
| CAS/Product ID | *Required fields | |
| Lot/Batch Number | *Required fields | |
Packaging of Trypan Blue
Industrial Trypan Blue Procurement FAQ
1. How do you ensure product quality consistency?
We rely on a mature China-based manufacturing system and implement strict batch control and quality inspection for every shipment. Our production process complies with ISO 9001 standards, and a COA is provided for each batch to ensure consistent and reliable quality.
2. Do you offer competitive pricing for bulk orders?
Yes, as a China-based manufacturer and bulk supplier, we provide tiered pricing based on order volume. We support FOB and CIF quotations, as well as L/C payment terms for large transactions to meet international trade requirements.
3. Can you support large-volume or long-term supply?
We have stable production capacity and a well-established supply chain, enabling continuous bulk supply. We support ton-level and container-level orders, as well as long-term cooperation projects.
4. What is your typical lead time?
For in-stock products, shipment can be arranged within 1–3 days. For bulk packaging or customized orders, lead time depends on the specific quantity and production schedule.
5. What documents do you provide?
We can provide complete documentation upon request, including COA, SDS, TDS, and other compliance certificates, meeting industrial procurement and export requirements.
6. Can you customize product specifications or packaging?
Yes, we support customization of parameters such as purity, impurities, residual solvents, and heavy metals. We also offer various bulk packaging options, including 25kg bags, drums, and IBC tanks, suitable for industrial applications and transportation.
7. What shipping options do you support?
We support both FCL (Full Container Load) and LCL (Less than Container Load) shipments, and can flexibly arrange logistics solutions based on order size to meet bulk chemical export needs.
8. Do you provide technical or application support?
Yes, we provide basic application guidance and technical support based on industry experience to help customers optimize product usage.
9. How do you handle compliance and export regulations?
We are familiar with international trade and export regulations, ensuring all products comply with relevant requirements. We also provide complete documentation to support smooth customs clearance and global delivery.
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